Collagen fibers in the native tissue form complex alignment patterns critical in guiding cell migration. While uniformly aligned fibers are known to facilitate migration through a process called contact guidance, the extracellular matrix (ECM) also contains spatial gradients in fiber alignment created by cell-generated traction forces. Although graded biophysical cell guidance cues such as soluble factors and stiffness are well-studied, the effect of graded alignment on cell migration remains largely unexplored. In this seminar, I will present a microfluidic biofabrication platform that uses controlled extensional flows to engineer 3D collagen hydrogels with precisely tunable fiber alignment landscapes. Our results show that alignment gradients provide a directional bias for single cells and cell aggregates compared to uniform fiber alignment domains. These findings suggest a new guidance cue and emphasize the mechanical role of extracellular matrix anisotropy in cell motility. This platform establishes a framework for integrating engineering approaches into the study of ECM-guided migration, relevant to tissue engineering, cancer research, and biomaterials design.